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    Home > Medical News > Medical Research Articles > PacBio Adds New Capabilities for Epigenomic Testing and Simplifies Sequencing

    PacBio Adds New Capabilities for Epigenomic Testing and Simplifies Sequencing

    • Last Update: 2022-05-17
    • Source: Internet
    • Author: User
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    PacBio Inc.
    today announced a new capability for the detection of DNA methylation using the Sequel IIe and Sequel II Sequencing Systems

    .
    Due to the limitations of traditional sequencing technologies, epigenome information is often unexplored, but the powerful HiFi sequencing technology breaks through this limitation and can obtain epigenome information

    .

    Using PacBio's single-molecule sequencing approach, researchers are able to detect modified bases in native DNA during standard HiFi sequencing, providing insights into the epigenome without additional time and cost
    .
    This advance will help them unlock new opportunities in a wide range of applications

    .

    According to PacBio, the update also includes extensive workflow improvements to enhance the customer experience, such as streamlining and accelerating library preparation processes and real-time monitoring of instrument performance
    .
    In addition, it supports the analysis of recombinant adeno-associated virus (rAAV) genome sequencing, a rapidly growing biopharmaceutical application associated with gene therapy and vaccine development research

    .

    Emily Farrow, Ph.
    D.
    , director of laboratory operations at Children's Mercy Hospital in Kansas City, said: "Multiple tests are currently required to evaluate patients with rare diseases.

    HiFi sequencing promises to change this, enabling the determination of genetic and epigenetic patterns across the entire genome in a single experiment.
    Epigenetic variation

    .
    We have successfully used HiFi sequencing in hospitals to identify aberrant methylation in repeat expansion cases and plan to apply it to all genomes sequenced in the future

    .
    "

    "We found that the CpG methylation patterns detected in the tomato and maize genomes using HiFi sequencing were highly consistent with standard bisulfite sequencing, while distinguishing other short Transposable elements and other sequences that cannot be obtained by read-length methods
    .
    ” He believes that with this capability, scientists can use a single data type to achieve ultra-high-quality genomic and epigenome analysis

    .

    Analysis of epigenomes has historically been difficult, sacrificing read length, accuracy, and completeness, and adding complexity to the pipeline
    .
    PacBio's sequencing technology automatically detects critical modified bases (5mC) in humans and multiple other species, providing a comprehensive view of the genome and epigenome without sacrificing read length or accuracy and requiring no additional manipulation

    .

    HiFi sequencing just got easier after streamlining the process
    .
    The newly launched SMRTbell prep kit 3.
    0 can reduce the time of whole genome sequencing by 50%, and reduce the amount of DNA input by 40%, such as only 3 μg DNA per human genome

    .
    PacBio also introduced new single-reaction sequencing plates and SMRT Cell 8M trays to allow customers to run samples better

    .

    Christian Henry, President and CEO of PacBio, said: "Our latest enhancements to the Sequel II and IIe platforms, including 5-base sequencing and improved workflows, promise to simplify the ability to generate high-quality data and help people gain a deeper understanding of the genome's complexity
    .
    "


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