Pattern edicom's proteomic fine map.

Diatom is an important class of single-cell photosynthesis eukaryotic organisms, widely distributed, providing about 20% of the earth's primary productivity, which is of great significance to the entire Earth biosphere.
triangular-brown-fingered algae (Phaeodactylum tricornutum), a model organism of marine diatoms, whose genome sequence was published in 2008, is still poorly annotated.
Proteogenomics is a deep commentary on genomes using proteomic data, especially high-precision series mass spectrometry data, combined with genomic and transcription data. Ge Feng, a researcher at the Institute of Aquatic Biology of the Chinese Academy of Sciences,
, used the research strategies and methods of protein genomics, completed the genomic depth analysis of model blue bacteria (PNAS, 2014,111 (52: E5633-E5642) and developed GAPP (Molecular and Proteomics.2016;15)3555) for pronuclear organisms.
on the basis of these work, Ge Feng's discipline group analyzed the genome of triangular brown-fingered algae in depth and constructed a detailed map of proteomics.
the subject group obtained the de-redundant triangular lignite genomics database by integrating multi-group data such as genomes, transcription groups, ESTs sequences, and the database, and by integrating sample pre-separation, bienzyme cutting and high-resolution mass spectrometry techniques based on protein and peptide segments. High-quality mass spectrometry data, mass spectrometry data identification integrated the results of a variety of search engines, improve the depth and coverage of protein identification, and adopted more stringent peptide segment false positive control strategy, so as to improve the credibility of the identification results, through the development of new algorithms, the discovery of new variable shears in eytranstrual organisms and the identification of point mutation genes.
the study accurately identified 6,628 anotated encoded genes; an in-depth analysis of unidentified commented genes found that 1,895 genes may not have encoded proteins; 606 new protein-coded genes were identified and 506 commented encoded genes were corrected, of which 56 newly discovered protein-coding genes were incorrectly predicted as long-chain non-coding RNA (LNCRNA); Micropeptides with important functions, 21 new variable shears, and corrections of 73 variable shear sites of the annotated genes and 58 genes with amino acid mutations;
through the completion of the above work, the deep annotation of the triangular brown-fingered algae genome was realized, and a detailed map of proteomics was constructed.
on the basis of the above work, the study also established a complete genome-based protein group fine mapping of experimental technology and analysis process, can be applied to a variety of sequencing eyreseutic organisms, become an important tool for interpreting the eyrenucleographic genome and its functional analysis.
research "Genome of the model a model diatom phaeod actylum tricornutum use an integrated proteogenomic pipeline" online published in Molecular Plant.
first author is senior experimental is Yang Mingkun, and the communication author is Ge Feng, the research was funded by the National Key Research and Development Program (2016YFA0501304).
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