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    Home > Biochemistry News > Biotechnology News > PCR Cloning of Human Immunoglobulin Genes

    PCR Cloning of Human Immunoglobulin Genes

    • Last Update: 2021-03-05
    • Source: Internet
    • Author: User
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    One of the first steps in an antibody-engineering project is the isolation of the immunoglobulin heavy (V
    H
    )- and light (V
    L
    )-chain variable-region genes that encode the binding domains of an antibody. This is best accomplished using the polymerase chain reaction (
    PCR
    ). Before PCR, cloning antibody genes was a laborious process, requiring the creation and screening of genomic or c
    DNA
    libraries. PCR has streamlined the process considerably, simplifying tasks such as the isolation of V
    H
    and V
    L
    genes from hybridomas, and allowing entirely new approaches such as generation of large antibody fragment gene repertoires from immunized or na�ve hosts that can be displayed on filamentous phage (antibody phage display;
    see
    Chapter 8 ) (
    1
    ) or in other display technologies (e.g., ribosome display;
    see
    Chapter 9 ).
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