PCR in the genetic diagnosis of viral infection
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Last Update: 2021-02-27
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Source: Internet
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Author: User
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total RNA extractionusing reverse transcriptase synthesis c
DNAPCR
reaction:reactor tissue: reaction buffertemplate (synthetic
cDNA
above)substrate (dATP, dGP, dG) TP, dCTP, dTTP) Sexuals (HIV-specific citants) TaqDNA polymerase 50 slcycle: 95 degrees C, 30sec (denaturation)55 degrees C, 1min (deflect) 30 cycles72 degrees C, 1min (polymerization)detection: electrophoresis or
nucleic acid
hybrid
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