Peking University Academy of Biological Sciences publishes two articles in a row to develop a new method for comprehensive evaluation of gene editing products

On August 8, 2021, Peking University's School of Life Sciences and Peking University-Tsinghua Life Sciences Joint Center Hu Jiazhi's group published online in Nucleic Acids Research entitled " Global detection of DNA repair outcomes induced by CRISPR-Cas9 " and " In-depth assessment of the PAM compatibility and editing activities of Cas9 variants " two research papers

The development of the CRISPR-Cas9 system for genome editing has changed the way of life science research, and at the same time has brought great potential for the treatment of diseases and the development of biotechnology

Figure 1.

In order to reduce the off-target activity of SpCas9 and broaden its target range, workers in this field have developed a variety of SpCas9 variants

Figure 2.

In summary, the work of these two articles systematically evaluated the DNA repair products in the CRISPR-Cas9 editing process and explained its influence mechanism, suggesting that we inhibit the potential mutagenic danger of non-homologous end joining (NHEJ) during gene editing.

Researcher Hu Jiazhi of Peking University is the corresponding author of the two articles