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    Home > Biochemistry News > Biotechnology News > Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification

    Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification

    • Last Update: 2020-12-15
    • Source: Internet
    • Author: User
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    The ability to directly manipulate the human genome to correct a disease-related mutation, introduce a sequence change thatwould lead to site-specific gene knockout, or increase gene expression is a very powerful tool with tremendous clinical value. Triplex formation by synthetic
    DNA
    -binding molecules such as peptide nucleic acids (PNAs) has been studied for over 20 yearsand much of the work in the last 10 years has shown its great promise in its use to direct site-specific gene modificationfor the use in gene therapy. In this chapter, detailed protocols are described for the design and use of triplex-forming PNAsto bind and mediate gene modification at specific chromosomal targets. Target site identification, PNA and donor oligonucleotidedesign, in vitro characterization of binding, optimization with reporter systems, as well as various methods to assess genemodification and isolate modified cells are described.

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