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Isolated photosynthetic materials have a relatively short active life time that limits their effective use. To circumvent this limitation, various immobilization techniques have been designed to improve their stability both under storage and working conditions. The immobilization methods are identified either as chemical or physical procedures depending on whether covalent bonds are established or not. In this chapter, two immobilization methods frequently used are described: a physical one based on the entrapment of photosynthetic materials in photo-crosslinkable poly(vinylalcohol) polymer bearing styrylpyridinium groups (PVA-SbQ) and a chemical one where the photosynthetic materials are immobilized by coreticulation in an albumin-glutaraldehyde cross-linked matrix (BSA-Glu). Different immobilization procedures in relation with various photosynthetic materials are mentioned.