Pig serum preparation techniques.

people working in animal husbandry and veterinary work at the grass-roots level know that disease surveillance of animals is often an important task. Epidemic surveillance requires blood harvesting and preparation ofserum . Due to the limitations of the grass-roots technical conditions, working environment and collection equipment, it is often the grass-roots veterinary personnel to separate from pig blood without bleeding.

this is common among all township and village animal husbandry and veterinary personnel. Today, cities and counties require townships to report animal serum every quarter.

some units can only deal with things. However, due to the reported pig serum is not qualified to complete the task well, often by the superior leader's rebuke. Indeed, it is difficult to prepare pig serum. However, as long as you have mastered some of these techniques, you will prepare a qualified pig serum in one go. If you are interested, please look down.

1. Collection purpose and object

1.1 used as a vaccine< a href=""> antibody monitoring to choose healthy pigs that have been vaccinated for testing and have been vaccinated for more than one week or more than three months.

1.2 is used for disease surveillance to blood all pigs in the jurisdiction. Generally each natural canton choose two or three points can be. Scale pig farms and out-of-village farming communities can be added 1-2 blood points.

1.3 used as a home seedling to mainly choose a healthy pig after one week of recovery from an epidemic or after more than a week of vaccination against an epidemic.

2." Blood collection site and method

2.1 piglets generally weigh less than 25 kg piglets, mainly collect blood in the front cavity veins. The method is: the assistant will sleep on the pig's back, with iodine wine to the pig's chest recesses disinfection, take sterile syringe so that the needle and the pig body with a 45-degree angle slowly pierced into 2-3cm, while stabbing into the side to do blood pumping action. In this way, it is easy to extract blood.

2.2 pigs, which typically weigh more than 25 kg, collect blood mainly from the ear veins. The method is: the assistant with a retainer to keep the pig standing, the player one hand fixed one side of the pig's ear, choose the ear of the thicker blood vessels with iodine wine disinfection, with a drop of hose needle into the vein, and then use test tube to collect the blood flow. Each test tube can collect 5 ml.

3. Serum's serum

put the test tube or syringe (note: first pull the syringe piston back a centimeter or two, to leave a gap in the bleeding) into a sink or porcelain tank with 40 to 45 degrees hot water, and at a angle of about 30 degrees. Hot water should be more, and at any time with < a href" > thermometertemperature measurement. If the temperature decreases, replace the new water so that the hot bath remains in the specified temperature at all time. Generally after 30-40 minutes, serum can be dissected.

if extracted with a syringe, it can also be placed on the hot head (of course, the temperature of the hot surface can not be too high or too low, generally around 50 degrees), and the piston back pull a centimeter or two, the needle end pad higher. Then cover it with a warm quilt or cotton pad, after half an hour to an hour or so serum can be dissected.

4. Reasons for serum failure

4.1 Most veterinarians collect too much at once while collecting blood, and then pour the collected blood into other containers.

4.2 hot bath temperature does not remain good.

4.3 sometimes picks up test tubes or other blood containers from hot trances or hot water baths several times to shake blood, causing local temperature instability.

5. Preparation of pig serum techniques

5.1 avoids hemolysis. After the blood sample is collected, do not shake, try to maintain a relatively stationary state, tilted in the test tube holder or water cup. If collected with a syringe, flatten it on a flat plate or in a glass of water.

5.2 timely warm-up. After all the blood samples have been collected, move them to a hot bath or hot tub.

5.3 keeps the temperature constant. If heating is carried out using a hot bath, a thermometer should be placed to observe the temperature changes. And at any time to add new hot water to maintain a constant temperature.

5.4 to observe serum analysis. Generally warm up after half an hour to observe once. Normally, a small amount of serum has been serumed at this time.