Plant histone H2A 95th serine phosphorylation modification point can regulate the flowering time of amoeba.

Ding Yong, a professor at the School of Life Sciences of the University of Science and Technology of China and the Center of Molecular Excellence of the Chinese Academy of Sciences, found that the plant histamine H2A 95th phosphate modification site, a plant-specific site, phosphorylated 95 serine, can regulate the flowering time of amoeba, as well as the abundance of histonin change H2A.Z.
results are phosphorylation of histone H2A serine 95:a plant-specific mark involved in flowering time regulation and H2A. Z deposition is published in the August 8 issue of The Plant Cell.
contain information about the growth and development of living individuals, which is functioned by different modification sites on hisoproteins and different hisoprotein variants.
animals, the individual life of a plant begins with a seed and is in an undifferentiated state.
if histone modification contains information about biological development processes, then there may be specific modification sites on histones in the plant and regulate their own unique growth and development processes.
studies have found that the phosphorylase MUT9P-LIKE-KINASE (MLK4) of the amoeba is capable of phosphorylated histamine H2A, the 95th bit of serine, which is found only in some algae and The spot was not found in moss, ferns,sub-plants and quitter plants in land-based plants, but in yeast, fruit flies or mammals (humans, mice), indicating specific histone spots in plants.
in the case of long sunlight, the missing mlk4 mutant has a dedicate of late flowers, while in the case of short sunlight, there is no obvious dedication, indicating that MLK4 is involved in the light cycle pathway.
studies have found that MLK4 can interact with MYB transcription factor CIRCADIAN CLOCK LINKEDED 1 (CCA1), which can take MLK4 directly to the GIGANTEA (GI) gene, complete 95 phosphorylation of histone H2A, and MLK4 can promote GI expression levels.
MLK4, which had a point mutation, lost kinase activity, it was not complementary to the physiome of the mlk4 mutant late flower, indicating that 95 phosphorylation of histrotein H2A could promote the flowering time of amoeba, as well as the intergenerational alternating process.
in animal cells, H2A phosphoric acid is mostly associated with DNA damage, while 95 phosphorylation of H2A promotes gene expression levels and has different functions than animal cell H2A phosphoric acid.
In order to analyze the relationship between 95 phosphorylation of H2A and gene transcription, further studies have found that MLK4 and chromosin remodeling complex Swi2/Snf2-Related ATPase (SWR1) and NuA4 have a common sub-yaf9a formation complex, and the functionally missing mlk4 reduces the transcription levels of GI genes by reducing the level of acetylation of histones H2A.ZH4.
the study found that the plant-specific histone H2A modification point and its function, providing an example of how histone modification regulates plant flowering time and the transformation between histone variants.
first author of the work of Su Yanhua, a fourth-year Shubo graduate student in the Dingyong group, and other authors include Wang Shiliang and Zhang Fei of the same group.
the study was carried out with the co-financing of the National Natural Fund, the Youth Thousand People Program of the Central Group Department, the Molecular Plant Pilot (Class B) of the Chinese Academy of Sciences and the China University of Science and Technology Microscale National Laboratory.
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