Plant Journal: Tomato lncRNA16397 and disease-causing mold resistance mechanism study.

1. The background-causing disease mold is a biotrophic pathogen and a pathogen of late tomato disease (LB).
LB has long been widespread in some countries, causing serious economic losses to tomato cultivation;
previous study of tomato pathogen interactions has found that different varieties of tomatoes exhibit different pathogen resistance.
advances in histological sequencing technology have helped identify thousands of long non-coding (lnc)RNAs in plant species, the role of lncRNA in plant-pathogen interactions remains largely undested.
2. Study objectives Solanum pimpinellifolium L3708 (Sp, P. infestans-resistant line) and S. lycopersicum Zaofen No.2 (Slz, P. infestans-sensitive line) tomato blades 3. The results of the study analyzed transcriptome sequencing data and identified a total of 1037 DEGs and 688 DELs between resistant and sensitive tomatoes.
network shows 128 DEGs and 127 DELs.
analysis, it was found that lncRNA16397 regulates its expression as an insoeverted transcript of SlGRX22, and induces SlGRX21 expression when lncRNA16397 is over-expressed.
addition, tomatoes that over-express lncRNA16397 and SpGRX have fewer symptoms of disease and lower accumulation of reactive oxygen (ROS) than wild tomatoes infected with disease-causing mold.
results show that lncRNA16397 in tomatoes induces SlGRX expression to reduce ROS accumulation and reduce cell membrane damage, thereby enhancing resistance to disease-causing mold.
(a) DEGs and DELs.
(b) Vinntu: DEG expressed in sp and Slz samples and specific expressions in a certain tomato variety.
level of 12 chromosomal lncRNA (log 10FPKM) in tomatoes.
are the two cocentric rings in the diagram, each corresponding to a different sample.
sp and Slz samples from the outside to the inside, respectively.
(d) the composition of different types of lncRNA.
of common and specific lncRNA between sp (e) Sp and Slz samples.
(color numbers can be viewed at wileyonlinelibrary.com) 4. The findings of this study provide an understanding of the lncRNA involved in tomato response to disease-causing mold infection, and have been experimentally demonstrated that the lncRNA16397-GRXs network is an important part of the tomato pathogenic mold network, providing ideas for the selection of highly biologically coercive resistant tomatoes.
5. Reference Cui J, et al. (2017) Comparative transcriptome analysis between a resistant and susceptible tomato allows the reality of lncRNA16397 conferring resistance to Phytophora infestans by co-expressing glutaredoxin. The Plant Journal. doi: 10.1111/tpj.13408 The lncRNA sequencing in this study was provided by Igawa Bio.