PloS One: Choose the right toothpaste and mouthwash to prevent new crowns!

New coronary pneumonia is a serious global public health problem caused by SARS-CoV-2

New coronary pneumonia is a serious global public health problem caused by SARS-CoV-2

As the saying goes, "Illness comes from the mouth," so does the new coronavirus

ACE2 and TMPRSS2 are in many kinds.

1.

1.

In order to understand the effects of commercially available toothpastes and mouthwashes on the mechanism of SARS-CoV-2 entry into host cells, we evaluated the inhibitory effects

In order to clarify the details of the inhibition and determine the IC50 value, the dose-response relationship of these five components was evaluated

In order to clarify the details of the inhibition and determine the IC50 value, the dose-response relationship of these five components was evaluated

2.

2,

Next, 30 ingredients were screened to test their inhibitory effects on TMPRSS2 serine protease activity

Next, 30 ingredients were screened to test their inhibitory effects on TMPRSS2 serine protease activity

In order to clarify the details of the inhibitory effect and determine the IC50 value, an IC50 value (w/w) of 0.

In order to clarify the details of the inhibitory effect and determine the IC50 value, an IC50 value (w/w) of 0.

3.

From the results, the most stable docking mode obtained in the docking study reproduces the crystal structure shown in Figs.

From the results, the most stable docking mode obtained in the docking study reproduces the crystal structure shown in Figs.
5A and 5B
.
Although the previous study used molecular operating environment (MOE) software to search for the binding site of the TMPRSS2 inhibitor camostat mesylate in the model, we conducted a docking simulation at the binding site of the crystal inhibitor
.
The docking study reproduced the crystal structure of the inhibitor, as shown in Figures 5C and 5D
.
According to the evaluation of the crystal structure of our TMPRSS2 model, it is found that the TMPRSS2 model is very similar to the crystal structure
.
All the side chain and main chain conformations of the binding site of our model inhibitor are very consistent with the side chain and main chain conformations of the human TMPRSS2 crystal structure, as shown in Figure S1
.
This strongly supports the results of the docking simulation using the predicted TMPRSS2
.
We also performed docking simulations using an improved human TMPRSS2 crystal structure (PDB ID: 7MEQ)
.

Based on the above conditions, a docking simulation was performed on the 7 test components
.
The results show that n-lauroyl-n-methyl taurine , n-lauroyl sarcosine, gluconic acid, dodecyl sulfate and (E)-tetradecene-1-sulfonic acid are compatible with human ACE2 Weak binding at the inhibitor binding site (Table 3, Figure 6A)
.
In addition, gluconic acid and transaminase may weakly bind to human TMPRSS2 at the inhibitor binding site ( Table 4 , Figure 6B ) .
These results indicate that these ingredients can function through the same basic mechanism .

Based on the above conditions, a docking simulation was performed on the 7 test components
.
The results show that n-lauroyl-n-methyl taurine , n-lauroyl sarcosine, gluconic acid, dodecyl sulfate and (E)-tetradecene-1-sulfonic acid are compatible with human ACE2 Weak binding at the inhibitor binding site (Table 3, Figure 6A)
.
In addition, gluconic acid and transaminase may weakly bind to human TMPRSS2 at the inhibitor binding site ( Table 4 , Figure 6B ) .
These results indicate that these ingredients can function through the same basic mechanism .

In summary, this study proved for the first time that toothpaste and mouthwash ingredients have an inhibitory effect on the key host factors that SARS-CoV-2 enters into host cells
.
The six common ingredients contained in commercially available toothpastes and mouthwashes showed inhibitory effects on the interaction of spurs single white-ACE2 and the protease activity of TMPRSS2
.
Because ACE2 and TMPRSS2 are essential for SARS-CoV-2 to enter the host cell, the five components that are effective for ACE2 and TMPRSS2 ( sodium dodecyl sulfate [ SDS], sodium tetrapolypropionate  [ TDS], methyl lauroyl cattle Sodium sulfonate [ LMT], Sodium N-gluconate [ LSS] and copper gluconate [ GCU]) may have a two-step inhibitory effect and have a high preventive effect on SARS-CoV-2 infection .
In addition, transamidation (TXA) inhibits TMPRSS2 protease activity .
Therefore, the results of this study show that toothpastes and mouthwashes containing these ingredients can help prevent SARS-CoV-2 infection .

In summary, this study proved for the first time that toothpaste and mouthwash ingredients have an inhibitory effect on the key host factors that SARS-CoV-2 enters into host cells
.
The six common ingredients contained in commercially available toothpastes and mouthwashes showed inhibitory effects on the interaction of spurs single white-ACE2 and the protease activity of TMPRSS2
.
Since ACE2 and TMPRSS2 are essential for SARS-CoV-2 to enter the host cell, the 5 components that are effective for ACE2 and TMPRSS2 ( sodium dodecyl sulfate [ SDS], sodium tetrapolypropionate  [ TDS], methyl lauroyl cattle Sodium sulfonate [ LMT], Sodium N-gluconate [ LSS] and copper gluconate [ GCU]) may have a two-step inhibitory effect and have a high preventive effect on SARS-CoV-2 infection .
In addition, transamidation (TXA) inhibits TMPRSS2 protease activity .
Therefore, the results of this study show that toothpastes and mouthwashes containing these ingredients can help prevent SARS-CoV-2 infection .



Sulfate [dodecyl sodium four polypropylene  [ four sodium poly methyl lauroyl taurate [ methyl lauroyl taurate [ N- Sodium Gluconate { N- [sodium gluconate copper gluconate [ Copper Gluconate【

 

Original source:

Riho Tateyama-Makino,et al,The inhibitory effects of toothpaste and mouthwash ingredients on the interaction
between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro.
PLOS ONE, September 17, 2021

https://doi.
org/10.
1371/journal.
pone.
0257705 

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