echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > PNAS: Exploring 'most deadly' lung cancer Scientists discover new application of CRISPR-Cas9 system

    PNAS: Exploring 'most deadly' lung cancer Scientists discover new application of CRISPR-Cas9 system

    • Last Update: 2020-06-06
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    However, this situation may soon be brokenA team of researchers at the Massachusetts Institute of Technology applied the CRISPR-Cas9 system to the study of targeted gene mutations in small cell lung cancer, and used it to discover that gene p107 plays a role in the SCLC anti-cancer gene, and the loss of p107 and its close relative p130 leads to a noticeable tumor phenotypeboth repeated mutations in about 6% of human SCLC tumorsThe researchers adapted the CRISPR-Cas9 system into a mature SCLC mouse model, and the feasibility of this method in SCLC was verified by simulating the p107 and p130 deficiencies in Trp53/Rb15.5 months after the tumor was developed, the researchers isolated genomic DNA from tumors in infected animals and then sequenced the corresponding sgRNAs-targeted genomic sites in a targeted depth sequencing, finding that the vast majority of sequences detected included transcoding or deletion, with each tumor having 1-4 Different mutant alleles, which demonstrated the feasibility of modeling candidate tumor suppressor gene deletions in SCLC using the CRISPR-Cas9 system, and proved that p107, like p130, was a functional tumor suppressor in SCLC(the loss of p107 accelerates tumor progression in SCLC)In further experiments, the researchers observed that animals infected with sgp107 also showed higher rates of metastatic lymph node metastasis in the advanced stages of cancer than animals infected with sgp130Compared with p130 deficiency, p107 deficiency led to a change in the distribution of early tumor lesions in the lungsThe researchers then increased the dying measurement of phosphating histone H3 (pHH3) to analyze the proliferation and apoptosis rates in two groups of animal tumorsThe results showed that advanced tumors from sgp107 infected animals showed higher rates of proliferationInterestingly, no such differences were observed in early tumorsExperimental data show that the loss of p107 has a significant effect on the development of SCLC compared with the loss of p130this study demonstrates the feasibility of simulating tumor suppression gene loss in SCLC mouse models using the CRISPR-Cas9 system, which opens the door to verifying the function of other candidate genes that frequently mutate in SCLC and will also help validate future Therapeutic Targets for SCLC.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.