Points for attention in the use of ultrasonic extraction unit of Shandong Baihe Company

Shandong Baihe Biotechnology Co.
, Ltd.
has been focusing on ultrasonic extraction and low-temperature evaporation and concentration production line equipment for more than 10 years.
Its good extraction quality and high-speed work efficiency have been welcomed by medical research, biopharmaceutical and other industries
.

But do you know some key points that should be paid attention to when using the ultrasonic extraction tank? Now let's take a look at the key points that should be paid attention to when using the ultrasonic extraction tank: 1.
Remember to be empty (the ultrasonic horn must be inserted into the sample before it can be turned on)
.

2.
The depth of the horn (ultrasonic probe) into the water: about 1.
5cm, the height of the liquid level needs to be more than 30mm, the probe should be centered, and do not stick to the wall
.

Ultrasound is a vertical longitudinal wave, and it is not easy to form convection if it is inserted too deep, which affects the crushing efficiency
.

3.
Ultrasonic parameter setting: Set the working parameters of the instrument (see the instruction manual for specific settings).
For samples that are sensitive to temperature requirements (such as bacteria), an ice bath is generally used externally.
The actual temperature must be lower than 25 degrees, and the protein nucleic acid will definitely not.
Transgender
.

1) Time: The ultrasonic time should not exceed 5 seconds each time, and the interval time should be greater than or equal to the ultrasonic time to facilitate heat dissipation
.

The time setting should be based on the principle of short ultrasonic time and many ultrasonic times, which can prolong the life of the ultrasonic vibrator and the probe
.

2) Ultrasonic power: it should not be too large to prevent the sample from splashing or foaming.
If the sample capacity is less than 10ml, the power should be within 200w, use a 2mm ultrasonic probe, and set the horn selection switch behind the panel to the corresponding gear; 10- The power of the sample capacity of 200ml is 200-400w, choose a 6mm ultrasonic probe, and set the horn behind the panel to the corresponding block; the power of the sample capacity above 200ml is between 300-600w, choose a 10mm ultrasonic probe, and put the horn at the back of the panel.
The horn hits the corresponding gear; (the power of the 2mm small probe should not exceed 350W)
.

3) Container selection: choose as many beakers as there are samples, which is also conducive to the convection of samples in ultrasound and improves the crushing efficiency
.

For example; 20ml processing volume requires a 20ml beaker
.

For example, setting parameters for 100ml E.
coli sample: ultrasonic for 5 seconds/interval for 5 seconds, 70 times (total time is 10 minutes), power 300W (for reference only), about 500ml, and the power is turned on to about 500W-800W
.

4.
If the sample is placed in a 1.
5ml EP tube, be sure to fix the EP tube to prevent the liquid level from falling after the ice bath melts, resulting in no load
.

5.
Daily maintenance: after use, scrub the probe with alcohol or perform ultrasound with water!