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Polygala saponins
Tenuifolin
【Structural formula】
【Molecular formula and molecular weight】C 36 H 56 O 12 ;680.
[Source] Polygala tenuifolia Willd.
【Properties】White powder
This product is soluble in methanol
【Purity Check】
TLC
1.
Developing solvent chloroform - methanol - water (6:3:0.
Inspection of 10% sulfuric acid ethanol solution, inspection under sunlight and ultraviolet light (365nm)
2.
Developing solvent ethyl acetate - ethanol - water (10:2:1)
Inspection of 10% sulfuric acid ethanol solution, inspection under sunlight and ultraviolet light (365nm)
High performance liquid chromatography
Chromatographic column C 18 , 5um (4.
Mobile phase methanol-0.
Detection wavelength 210nm
【Structural identification】205
3415, 2949, 2625, 1704, 1470, 1390, 1257, 1172, 1075, 1044, 1019
ESI-MS m/z: 679 [MH] -
1 H-NMR (C 5 D 5 N) δ: 4.
13 C-NMR (C 5 D 5 N) δ: 44.
【Storage】Room temperature
Related links: 3,6'-dierucyl sucrose