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    Home > Polymer: a new fluorescent polymer with low toxicity and pH control is expected to produce cytoplasmic targeted probes

    Polymer: a new fluorescent polymer with low toxicity and pH control is expected to produce cytoplasmic targeted probes

    • Last Update: 2017-11-21
    • Source: Internet
    • Author: User
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    Cell is the basic functional unit of any life form It is one of the most challenging tasks for chemists and biologists to have a deep understanding of cytochemistry Fluorescence imaging, especially specific organelle imaging, has become an important technology to understand cell proliferation, migration, division, fusion and lysis Fluorescence imaging technology has become a powerful technology, which enables biological researchers to track cell processes systematically and continuously At present, a variety of fluorescent materials have been developed for cell imaging, such as nanoparticles, organic dyes, fluorescent proteins But there are still some limitations in practice For example, inorganic small molecules may have the potential to cause heavy metal toxicity So far, most of the commercially available organic dyes are fluorescent small molecules, such as mitotrackerred cmxros, DAPI Although they are organelle targeted probes (mitochondria and nuclei), their fluidity, instability and toxicity in the process of cell imaging are still a problem In this regard, there have been a lot of research efforts to break this dilemma, such as combining fluorescent components with some materials with good biocompatibility Poly (aa-tpa-d) synthesis route (source: polymer) in recent years, polymer materials have attracted more and more attention in the biomedical field due to their unique optical properties, ease of manufacturing and imaging, and aggregation induced emission (AIE) characteristics It has many potential application characteristics, such as adjustable brightness, good biocompatibility, stable physical properties and biodegradation In some previous studies, polyacrylic acid (PAA) is a kind of polymer with great potential PAA has an ionizable hydrophilicity, and its swelling behavior is greatly dependent on pH It is because of its hydrophilicity and pH response that its application in biological field is possible The particle size distribution and SEM images of poly (aa-tpa-d) at different pH (source: polymer) were designed and synthesized by a research group led by Professor Ma Hengchang and Lei Ziqiang from the school of chemistry and chemical engineering, Northwest Normal University of China Poly (aa-tpa-d) was designed and synthesized with triphenylamine (TPA) derivatives as fluorescent nucleus and polyacrylic acid (PAA) as skeleton And study its application in biological imaging The mechanism of fluorescence change at different pH (source: polymer) in the design and synthesis of polymers, the research team first generated important intermediates n, N-bis (4-bromophenyl) - 4 '- Vinyl - [1 through Suzuki reaction of tris (4-bromophenyl) amine and (4-vinylphenyl) boronic acid, 1 '- biphenyl] - 4-amine (nbva), then copolymerization between nbva and AA was carried out to obtain the precursor polymer poly (aa-nbva) Finally, poly (aa-tpa-d) was obtained by the Heck reaction between poly (aa-nbva) and methyl acrylate In addition, (2e, 2'e, 2'e) - trimethyl 3, 3 ', 3' - (nitrilotris (benzene-4, 1-diyl)) triacrylate (tnbt) was synthesized for FT-IR spectral comparison study to determine the functional groups on the polymer surface The number average molecular weight (MN) and polydispersity index (PDI) of the polymer were determined by GPC After poly (aa-tpa-d) staining, A549 cell fluorescence image (50 μ g / ml) (source: polymer) in the following study found that the polymer poly (aa-tpa-d) synthesized by combining pH responsive PAA with AIE active TPA derivatives has good water solubility and biocompatibility, as well as pH responsive fluorescence change The designed target polymer can be used to generate the terrain hydrogel by hydrogen bonding and the interaction of methyl acrylate group C =O Considering the excellent fluorescence performance and low cytotoxicity of poly (aa-tpa-d), the material can be used as a biological probe for cell imaging Obviously, poly (aa-tpa-d) can be easily internalized and absorbed by mpc5 cells due to its low cytotoxicity, and it has been found that it has cytoplasmic specificity Therefore, poly (aa-tpa-d) has shown great potential as a cytoplasmic targeting probe In addition, the development strategy of combining the biocompatible PAA with AIE active fluorescein also provides creative suggestions for the design of some new biocompatible probes in the future Profile of Professor Ma Hengchang https://www.nwnu.edu.cn/aarticle.do? Id = 14111 profile of Professor Lei Ziqiang https://www.nwnu.edu.cn/aarticle.do? Id = 14063 paper link: http://www.sciencedirect.com/science/article/pii/s003238611731087x
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