echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Food News > Food Articles > Potato haploid induction line was successfully created

    Potato haploid induction line was successfully created

    • Last Update: 2022-11-15
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    Recently, the team of Huang Sanwen of the Shenzhen Institute of Agricultural Genomics, Chinese Academy of Agricultural Sciences created a potato haploid induction line, and constructed a method to obtain a high-purity diploid potato inbred line using bihaploid technology, which provides a strong technical guarantee
    for diploid potato hybrid breeding.
    The relevant research results were published in the Journal
    of Biotechnology (aBIOTECH).
     
    The traditional method of obtaining diploid potato inbred lines generally requires multi-generation self-inbreeding, long cycle and low efficiency, while haplo species can achieve pure line creation only through haploid induction and doubling two generations, which significantly accelerates the breeding process and efficiency of inbred lines, which is a common key technology
    in agricultural biological breeding.
     
    The researchers combined with FAST-Red fluorescent screening markers through the CRISPR/Cas9 system to construct a biallele mutant
    of the StDMP gene.
    On this basis, nearly 100% homozygous diploid potato material
    was obtained by hybridization, fluorescence screening, molecular marker identification, flow cytometry determination, and colchicine doubling.
    Compared with the traditional self-inbred method, this method does not have to overcome the structural barriers of potato inbred incompatibility, and greatly shortens the breeding cycle, which can be completed in about 8 months, which will provide a new solution
    for rapid potato breeding.
     
    The project was supported
    by the National Natural Science Foundation of China, the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences, and the Guangdong Provincial Major Project for Basic and Applied Basic Research.
     
    Original link:
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.