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    Home > Food News > Food Articles > Potato resistance mechanism to low-temperature saccharification revealed

    Potato resistance mechanism to low-temperature saccharification revealed

    • Last Update: 2021-04-17
    • Source: Internet
    • Author: User
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    Potato resistance mechanism to low-temperature saccharification revealed




    Potato resistance to low-temperature saccharification revealed

     

    French fries and potato chips are the most popular and high value-added potato processed products.
    Low-temperature storage of potato tubers leads to the accumulation of reducing sugars (glucose and fructose), which is called "low-temperature saccharification".
    The accumulation of reducing sugars seriously affects processing quality and healthy consumption.
    Resistance to low-temperature saccharification is an important trait in potato processing quality breeding, but the regulation mechanism of low-temperature saccharification resistance is still unclear.

    Recently, the potato team from the School of Agriculture and Biotechnology of Southwest University published a research paper online in Horticulture Research, revealing the mechanism of potato resistance to low-temperature saccharification.

    The previous research of the research group found that the invertase inhibitor StInvInh2 has a genotype-dependent low-temperature expression pattern, and low-temperature induced expression in low-temperature glycation-resistant genotype tubers mainly contributes to low-temperature glycation resistance.
    This genotype-dependent expression has no obvious relationship with post-transcriptional level regulation and gene promoter sequence polymorphism.

    StInvInh2

    In this study, the AP2/ERF VII transcription factor StRAP2.
    3
    and StInvInh2 were co-expressed from the perspective of transcription regulation .
    Through the analysis of gel migration block and dual luciferase reporter system, it was confirmed that
    StRAP2.
    3
    specifically binds to the ACCGAC element in the StInvInh2 promoter and activates its expression.
    Further using RNAi technology to interfere with
    StRAP2.
    3
    in potato low-temperature saccharification resistance genotypes specifically inhibited the expression of StInvInh2 in low-temperature storage tubers , and reduced the low-temperature saccharification resistance and processing quality.
    In contrast, in the low-temperature saccharification sensitive genotypes overexpressed
    StRAP2.
    3
    increased StInvInh2 expression, enhance the cold sweetening resistance and improve processing quality.

    StRAP2.
    3
    StInvInh2 StRAP2.
    3
    StInvInh2 StRAP2.
    3
    StInvInh2 StRAP2.
    3
    StInvInh2

    In summary, the study established a model of StRAP2.
    3-StInvInh2
    regulating low-temperature glycation resistance, decrypted the transcription regulation mechanism of StInvInh2 during low-temperature storage of tubers , and provided new insights for the study of potato low-temperature glycation resistance mechanism Improve the breeding process of potato low-temperature saccharification resistance.
    (Source: China Science News Zhang Qingdan)

    StRAP2.
    3-StInvInh2
    StInvInh2

    Related paper information: https://doi.
    org/10.
    1038/s41438-021-00522-1

    https://doi.
    org/10.
    1038/s41438-021-00522-1
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