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PPAR-Alpha Cloning, Expression, and Characterization

 Last Update: 2020-12-04
 Source: Internet
 Author: User

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metabolic genes

ppar alpha agonist

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Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that alsocomprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulationof many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number ofmarine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methodsfor cloning of PPARα genes by RT-
PCR
and RACE approaches and related protocols for studying the expression of cloned PPARαc
DNA
s in mammalian cell systems.

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