Precautions during column activation and maintenance

The column is the most important component of the high performance liquid chromatograph, whether the measured substance can be well separated and measured, the performance of the column plays a decisive role, therefore, special attention should be paid to the activation of the column, in addition, the daily maintenance of the column is also very important, effective maintenance can extend the service life

Activation of columns

The new liquid chromatography column is kept in a certain preservation solution, the so-called activation is to replace the preservation solution in it, rinse off other impurities that may remain in the column, so as not to affect the subsequent analysis work

There is a certain procedure for the maintenance of the column: the balance of the column, the regeneration of the column, and finally the maintenance

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Precautions for the use of liquid chromatography columns

The liquid chromatography column has no special instructions and is a mobile phase