Preparation method for PBS and D-PBS buffer solutions.

. PBS is a buffer solution commonly used in
biochemistry
that impedes pH changes in the solution, which contains sodium chloride, phosphate, phosphate, potassium chloride and potassium phosphate. Group concentration: 137 mM sodium chloride, 2.7 mM potassium chloride, 10 mM sodium phosphate, 2 mM potassium phosphate. This paper summarizes the preparation methods of PBS and D-PBS solutions.. 1. Regular PBS phosphate buffercomposition:potassium dihydrohydrogen 34 g 1 mol/L sodium hydroxide solution 175 mldistilled water 825 ml PH 7.2preparation:
dissolves phosphates in 500 mL distilled water, corrects pH with a solution of 1 mol/L sodium hydroxide, and dilutes them to 1000 mL with distilled water.dilution:1.25 mL of storage fluid, diluted to 1000 mL with distilled water. It is divided into 100 mL per bottle or 10 mL per tube, 121 degrees C
15 min
autocultension.:is mainly used to regulate pH without affecting experimental reactions in some chemical experiments, so that the experimental chemical reactions are carried out under optimal conditions. Partly used in food or feed industry processing, such as in the application of Elisa methods in food or feed
filter
toxin testing or in diluted buffers in food
microbial
testing.. 2. PBS Buffer preparation stepsconcentration:
137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4Preparation:
1 LPreparation Method: (1) Weighing the following
Reagents
, placed in 1 L
Berries
..NaCl 8 gKCl 0.2 g Na2HPO4 1.42 g KH2PO4 0.27 g (2) Add about 800 ml of deionized water to the beaker and stir to dissolve. (3) drop-plus hydrochloric acid to adjust the pH to 7.4, and then add deionized water to the solution to 1 L. (4) high temperature autobaric sterilization, room temperature preservation. note:
priceless cations in the PBS Buffer above, 1 mM CaCl2 and 0.5 mM MgCl2 can be added to the formula if required. . 3. D-PBS is a Duchy phosphoric acid buffer whole name: Dulbecco's Phosphate Buffered Saline
Group Molecular Weight Concentration (gm/L) Molar Concentration (mM) Potassium chloride (KCl) 750.202.67 hydrophosphate (KH2PO4) 1360.201.47 sodium chloride (NaCl) 588.00138.00 sodium phosphate (Na2HPO4) 1421.158.10 pH is 7.4. (1) Dulbecco's Phosphate Buffered Saline (D-PBS) is also a phosphate buffer, which differs from conventional PBS in that it contains slightly lower levels of phosphate and contains calcium and magnesium ions. D-PBS is mainly used in embryology research, mostly for embryonic flushing, freezing fluid and
culture
fluid. (2) D-PBS formula: sodium chloride 8 g, potassium chloride 0.2 g, phosphate d.15 g, potassium phosphate 0.2 g, calcium chloride 0.1 g, magnesium chloride containing 6 crystalline water 0.1 g dissolved in 1 L ddH2O. (3) above is formulated as D-PBS containing calcium and magnesium ions, or D-PBS without calcium chloride and magnesium chloride. (4) on the basis of the above formula, can also be added glucose, sodium acetone, etc., in order to be more suitable for the growth of culture. . D-PBS is a duchy phosphate buffer, is also a phosphate buffer, the biggest difference with conventional phosphate buffer is that the phosphate content is slightly lower, according to whether calcium and magnesium are divided into two kinds, namely D-PBS w/calcium and D-PBS w/o calcium
.