Preparation of Direct, Enzyme-Labeled DNA Probes
-
Last Update: 2021-03-02
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
The direct labeling of nucleic acid probes with horseradish peroxidase (HRP) was first described by Renz at European Molecular Biology Laboratory (EMBL) in 1984 (
1
). The methodology was combined with enhanced chemiluminescence (
2
) (a light-producing HRP catalyzed reaction based on luminol) (
see
Chapter 26 ) allowing the detection of specific hybrids on membranes (
3
). Further development led to the availability of the first light-based nucleic acid detection system; this was capable of detecting 2.5 pg of target nucleic acid on genomic Southern blots (
4
). Subsequent protocol and reagent improvements now enable researchers to reliably detect 0.5 pg of target nucleic acid. Recent advances have now elucidated an analogous system for the labeling of
DNA
probes with alkaline phosphatase (AP), offering even higher sensitivity when combined with the new dioxetane based chemiluminescence substrates (
see
Chapter 26 ).
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.