echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Preparation of phosphate buffer (PB).

    Preparation of phosphate buffer (PB).

    • Last Update: 2020-10-17
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

    1. A solution (0.2M sodium dihydrophosphate): NaH2 PO4 - H2 O 27.6g, dissolved in distilled water, diluted to 1000ml.

    2. B solution (0.2M hydrophosphate disodium water solution): Na2 HPO4 , 7H2 O 53.6g (or Na2 HPO4 , 12 H2 O 71.6g or Na2 HPO4 , 2 H2 O 35.6g) plus distilled water dissolved, add water to 1000ml.

    3. The preparation of different pH phosphate buffer (PB) buffers

    A liquid X ml (see table below), add B liquid Y ml, 0.2M petabytes. If distilled water is added to 200ml, it will be 0.1M PB.

    >
    PH
    .
    45.0.
    90.0
    < "top" width "55" >
    6.0
    < .
    15.0
    7.4
    6.3
    .
    <7.5
    13.0
    10.0
    < "td valign" "top" width "55" >
    6.7
    .
    93.0
    X ml
    Y ml
    pH
    X ml
    Y ml
    5.7
    93.5
    6.5
    6.9
    55.0
    5.8
    92.0
    8.0
    7.0
    39.0
    61.0
    5.9"
    10.0
    7.1
    33.0
    67.0.
    "60"
    12.3
    7.2
    28.0
    72.0
    6.1
    85.0
    7.3
    23.0
    77.0
    6.2.
    81.5
    18.5
    19.0
    81.0
    77.5
    22.5
    16.0
    84.0
    6.4
    73.5.
    26.5
    7.6
    87.0
    6.5
    68.5
    31.5
    7.7
    90.0
    6.6
    62.5
    37.5.
    7.8
    8.5
    91.5
    56.5
    43.5
    7.9
    7.0
    6.8
    51.0
    49.0
    8.0.
    5.3
    94.7


    if first assigned 0.1M, 1/15M and 1/200M A Liquid and B liquid, you can press the table to match the corresponding concentration of pH PB

    .
    pH (M/10) )
    < td val.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.
    Related Articles

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent Echemi's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.
    KH2 PO4 or NaH2 PO4 H2 O (ml)
    Na2 HPO4 (ml)
    pH (M/15)
    pH (M/200)
    46
    .