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1. A solution (0.2M sodium dihydrophosphate): NaH2 PO4 - H2 O 27.6g, dissolved in distilled water, diluted to 1000ml.
2. B solution (0.2M hydrophosphate disodium water solution): Na2 HPO4 , 7H2 O 53.6g (or Na2 HPO4 , 12 H2 O 71.6g or Na2 HPO4 , 2 H2 O 35.6g) plus distilled water dissolved, add water to 1000ml.
3. The preparation of different pH phosphate buffer (PB) buffers
A liquid X ml (see table below), add B liquid Y ml, 0.2M petabytes. If distilled water is added to 200ml, it will be 0.1M PB.
X ml | Y ml | pH | . X ml | Y ml |
5.7 | 93.5 | 6.5 | 6.9 |
55.0
5.8
92.0
8.0
7.0
39.0
61.0
5.9"
90.0
10.0
7.1
33.0
67.0.
6.0
"60"
12.3
7.2
28.0
72.0
6.1
85.0
15.0
7.3
23.0
77.0
6.2.
81.5
18.5
7.4
19.0
81.0
6.3
77.5
22.5
16.0
84.0
6.4
73.5.
26.5
7.6
13.0
87.0
6.5
68.5
31.5
7.7
10.0
90.0
6.6
62.5
37.5.
7.8
8.5
91.5
6.7
56.5
43.5
7.9
7.0
93.0
6.8
51.0
49.0
8.0.
5.3
94.7
if first assigned 0.1M, 1/15M and 1/200M A Liquid and B liquid, you can press the table to match the corresponding concentration of pH PB
KH2 PO4 or NaH2 PO4 H2 O (ml) | Na2 HPO4 (ml) |
pH (M/15)
pH (M/200)
46
. 
