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Preparation of Templates for Production of Ribozymes and Substrates

 Last Update: 2021-01-03
 Source: Internet
 Author: User

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molecular biology books

transcription mediated amplification

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DNA
dependent bacteriophage RNA polymerases has become a very powerful technique in molecular biology. Single-stranded RNAs generated by in vitro transcription can be used as substrates in a variety of experiments ranging from splicing and translation to structural studies. This chapter describes methods for the preparation of templates suitable for in vitro transcription.

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