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Tris - hydrochloric acid buffer (0.05M , 25 degrees C) 50 ml 0.1M trihydroxymethymethane (Tris) solution mixed with X ml 0.1N hydrochloric acid, diluted to 100 ml.
| X (ml) | ||
7.10 7.20. 7.30 7.40 7.50 7.60 7.70 7.80 7.90 8.00 | 45.7 44.7 43.4 42.0 40.3 38.5 36.6 34.5 32.0 29.2 | . < td valign," "top" width, "142" >
26.2
22.9
19.9
19.9
17.2
14.7
12.4
10.3
8.5
7.0
. trihydroxy methamethane (TrisCH2OH
C
HOCH2NH2
molecular weight of 121.14;
. 0. The 1M solution is 12.114 g/L. Tris solutions absorb carbon dioxide from the air and use the bottle tightly.
(Responsible Editor: King Han Kunlun).