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    Home > Biochemistry News > Biotechnology News > Preparation of Tris-hydrochloric acid buffer (0.05M, 25 degrees C).

    Preparation of Tris-hydrochloric acid buffer (0.05M, 25 degrees C).

    • Last Update: 2020-10-28
    • Source: Internet
    • Author: User
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    . < td valign," "top" width, "142" >
    8.10
    8.20
    8.30
    8.40
    8.50
    8.60
    8.70
    8.80
    8.90
    Tris - hydrochloric acid buffer (0.05M , 25 degrees C)
    50 ml 0.1M trihydroxymethymethane (Tris) solution mixed with X ml 0.1N hydrochloric acid, diluted to 100 ml.

    pH
    pH
    X (ml)
    X (ml)
    7.10
    7.20.
    7.30
    7.40
    7.50
    7.60
    7.70
    7.80
    7.90
    8.00
    45.7
    44.7
    43.4
    42.0
    40.3
    38.5
    36.6
    34.5
    32.0
    29.2
    < td valign""top" width"142" >
    26.2
    22.9
    19.9
    17.2
    14.7
    12.4
    10.3
    8.5
    7.0
    .
    trihydroxy methamethane (TrisCH2OH
    C
    HOCH2NH2
    molecular weight of 121.14;
    .
    0. The 1M solution is 12.114 g/L. Tris solutions absorb carbon dioxide from the air and use the bottle tightly.
    (Responsible Editor: King Han Kunlun).
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