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    Home > Chemicals Industry > Chemical Technology > Pretreatment of pyrazolone residue analysis technology-extraction method (2)

    Pretreatment of pyrazolone residue analysis technology-extraction method (2)

    • Last Update: 2021-09-30
    • Source: Internet
    • Author: User
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    B.
    Acidic drugs

    a.
    Neutral extraction

    Phenylbutazone and oxybutazone have low polarity, and there are many reports of direct extraction with organic solvents
    .


    Organic solvents can also deproteinize while extracting drugs


    Marti et al.
    used acetonitrile to extract phenylbutazone from plasma, and the supernatant was directly analyzed by HPLC
    .


    With betamethasone as the internal standard, the recovery rate was 83%, and the LOD and LOQ were 0.


    Dowling et al.
    used acetonitrile to extract phenylbutazone and other drug residues in milk.
    The acetonitrile extract was dried and re-dissolved in n-hexane, and determined by gas chromatography-tandem mass spectrometry (GC-MS/MS)
    .


    The CCa of phenylbutazone is 0.


    Pang Guofang and others determined the residues of phenylbutazone in the muscles of cattle, pigs, sheep, and chickens, extracted with methanol-0.
    25mg/mL dithiothreitol in ethyl acetate solution (1+7, v/v), and dithiothreose Alcohol acts as a stabilizer, and the extract is purified by solid phase extraction and then measured with an HPLC-ultraviolet detector
    .


    The method recovery rate was 89.


    b.
    Acidic extraction

    Butysone and oxybutazone are acidic, and the samples are more easily extracted into water-immiscible organic solvents after acidification
    .

    Marunaka et al.
    measured phenylbutazone and oxybutazone in blood and urine
    .


    Using γ-hydroxyphenylbutazone as the internal standard, the sample was first acidified with hydrochloric acid and extracted with benzene-cyclohexane (1+1, v/v).


    2) Ultrasonic assisted extraction (UAE)

    Ultrasonic extraction utilizes the alternating period of positive and negative pressure existing in the ultrasonic transmission process to generate sonic cavitation between the extraction solvent and the sample.
    The formation, growth and burst compression of bubbles in the solution disperse the solid sample and increase the contact between the sample and the sample.
    The contact area between the extraction solvents improves the transfer of the target from the solid phase to the liquid phase
    .


    Gentili et al.


    3) Molecular ultrafiltration extraction

    Molecular filtration extraction is to use a semi-permeable membrane to filter according to the molecular weight of each substance in the sample to achieve the purpose of extraction and purification.
    It filters out impurities such as proteins and amino acids with larger molecular weights in the liquid sample.
    Purify by filtering through membrane
    .


    De Veaul determines free phenylbutazone in bovine plasma.


    Related links: Pretreatment of pyrazolone residue analysis technology-extraction method (1)

     

     

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