Principle of super clean worktable

The principle, use and maintenance of super clean working table is to provide aseptic operating environment for laboratory work, to protect the experiment from the impact of external environment, and to provide some degree of protection for the external environment to prevent pollution and protect the operator Compared with the simple aseptic cover, the super clean platform has the advantages of allowing the operator to move freely, easy to reach anywhere in the operation area and high safety At present, the super clean worktable used in our country has imported products, such as biogard b40-112 This is a super clean worktable that meets the safety standard of class II A in the United States It is a product recommended for biological tests with low and medium risks (1) principle: the clean environment of the super clean working table is formed by the flow of clean air (in filter air) in the set direction in a specific space According to the air flow direction, the existing super clean table can be divided into vertical type, from the inside out type and lateral type Considering the operation quality and the impact on the environment, the vertical type is superior The clean air provided by the air supply filter plate descends through the operation area at a specific speed and is separated in the middle of the operation area It is sucked by the front air suction hole and the rear suction window The air inhaled in the lower part of the operation area is mixed with the air inhaled in the front and rear parts, and is pumped into the rear positive pressure area by the blower In the upper part of the machine, 30% of the air is discharged from the top through the exhaust filter plate, about 70% of the air is discharged The body re enters the operation area through the oxygen supply filter plate In order to supplement the air discharged from the exhaust port, the same volume of air is supplemented from the room air through the operation port These air will not enter the operation area, but form an air barrier Many domestic ultra clean worktables only have air supply filter plate, and the filtered air enters into the operation area to form a certain positive pressure The air is discharged into the ambient air from the set vent hole and operation port This air flow mode has no protection effect on the surrounding environment and the operator (II) the ultra clean bench where the commissioning is located shall be strictly tested before delivery to ensure normal use But this is not to say that the manufacturer's test can replace the necessary inspection and debugging before the operator uses it Because the environment of ultra clean worktable is different, only after proper inspection and debugging, can the equipment play a role in a large limit Choose a better environment for the machine before debugging It should be placed in a sterile room with air disinfection facilities If the conditions are not available, the machine should be placed in a clean room with less walking Adjust the height of each foot to ensure the safety and level of the operation surface A special circuit shall be adopted for the power supply of super clean workbench to avoid the change of air flow rate caused by circuit overload Ultraviolet germicidal lamp and fluorescent lamp for lighting are the standard configuration of ultra clean working table The blower provides the power for air flow It is clear whether these parts work normally It is difficult and important to check the air filter plate and its tightness, which is directly related to the normal use of the machine The simple examination method is nutrient agar plate method The newly purchased and unused ultra clean worktable can be fumigated in addition to being irradiated by ultraviolet lamp, and then an open nutrient agar plate is placed at the four corners and central position of the operation area when the machine is in working state The two-hour back cover is placed on the top of the cover and placed in a 37 ℃ incubator for 24 hours to calculate the colony number The average number of colonies per plate must be less than 0.5 (III) before using the ultra clean worktable, use ultraviolet lamp to irradiate for 30-40 minutes, and check that all kinds of openable doors and windows around the operation area are in the working position The operation is carried out in the center of the operation area, which is a safer area in design Before operation, we should have a preliminary understanding of the experimental materials, and understand the performance and safety level of the equipment we use Strictly implement the laboratory safety regulations Safety assessment must be carried out for the use of specific pathogens in any super clean working table If the experimental material will cause environmental pollution to the surrounding environment, it should be avoided to use in the model without exhaust filter plate, because the operation in the flowing air is the same as the detoxification Any advanced equipment can't guarantee the success of the experiment The purpose of using the super clean working table of animal quarantine laboratory is asepsis and avoiding cross contamination, so skilled operation and clear asepsis essentials are essential (4) the maintenance of ultra clean worktable is a more precise electrical equipment, and it is very important to carry out regular maintenance and repair First of all, keep the room dry and clean Humid air will not only rust the manufacturing materials, but also affect the normal operation of electrical circuits Humid air is also conducive to the growth of bacteria and mold Clean environment can also extend the service life of the filter plate In addition, regular cleaning of equipment is an important part of normal use Cleaning shall include routine cleaning and regular fumigation before and after use When fumigating, all gaps shall be completely sealed Cleaning shall include routine cleaning and regular fumigation before and after use During fumigation, all gaps shall be completely sealed If the operation port is equipped with a movable baffle cover type ultra clean workbench, it can be sealed with plastic film The filter plate and UV sterilization lamp of ultra clean worktable have a calibrated service life and should be replaced on schedule.