Principles and operating procedures of amino acid paper lithography.

relevant1.
(
chromatography): is a physical separation method. It is the use of the mixture of the physicochemical properties of the components, so that the components are distributed in varying degrees in two phases, one phase is fixed (called fixed phase), the other phase flows this fixed phase (called flow phase) and the components move at different speeds, so as to achieve separation.
2. Several commonly used
(1) adsorption layering (2) ion exchange layering (3) thin layer analysis (4) gel layering (gel
filtration
) (5) affinity layer The
(6) distribution of layering (suitable for soluble mixtures in both water and organic solvents)
when a substance is oscillat in two non-soluble solvents, it will be unevenly distributed in both phases. When equilibrium is reached, the ratio of the concentration of the substance in the two solvents is a constant, i.e. the distribution coefficient.if the distribution coefficients of the parts of a mix are sufficiently different in these two phases, they can be separated.
3.
the polarity of
amino acids and the structure of each amino acid , the experimental purpose1. Through the separation of amino acids, the basic principles and operating methods of paper
2. Mastering the operation technique of the amino acid paper upper layering method (dot sample, balance, spread layer, color display, identification II, experimental principle paper layering method is a kind of distribution layering, with filter paper as an inert support. The layering solvent (expansion agent) consists of organic solvent and water.
water on paper is adsorbed between cellulose fibers to form a fixed phase. Because the hydroxyl on cellulose is hydro-hydrogenic and is connected to water by hydrogen bonds, making this part of the water less prone to diffusion, it is still similar to the two phases that can be mixed with water solvents. When the organic phase flows along the paper through the analysis point, the solute on the analysis point is distributed between the water phase and the organic phase, a part of the solute leaves the origin and moves with the organic phase into the insoluble area, then re-distribution, a part of the solute from the organic phase into the water phase. As the organic phase continues to flow, the solute moves in the direction of the organic phase flow and is constantly distributed. The distribution coefficients of the various parts in the solute are different and the movement rate is different, so they can be separated from each other. The position of the substance on the paper layer map after it is separated is expressed as the RF value (ratio shift).the R-f value of a substance
certain
conditions is constant. The
of
value of R
f is related to the structure, properties, solvent system,
P
H layering filter paper and other factors. Amino acids, sugars, nucleic acids, steroids
hormones
, vitamins,
antibiotics
and many other substances can be separated by paper layering. Upper layer analysis, lower layer analysis, one-way layer analysis, two-way layer analysis. , instruments, materials and
reagents instruments: each group of students prepare a ruler, scissors or knife
1. Layering cylinders,
training
dishes, cartridges, small beakers, long-neck funnels, capillary tubes, hair dryers, electric blowers
drying
boxes, laminate filter paper, gloves, transparent tape.
of experimental materials and reagents
1. Standard amino acid solutions are made with 0.5% Lys, Ala, Ile, Met solutions
2. The mixture of amino acid solutions to be separated
3. Coloring agent 0.1% hydrationtritone acetone solution.
4. Extender (layering agent): This experiment uses acid solvent system
acid solvent system: orthotinol: 80% foric acid: water: 15:3:2 (volume ratio), the balance solvent and the expansion agent is the same. .