Procedure for determination of residues of α-Trenbolone, β-Trenbolone, 19-ethylene nortestosterone and epi-19-ethylene nortestosterone in cow urine

8.
2.
5.
6 Determination

(1) Liquid chromatography conditions

Chromatographic column: InertsilC ₁₈ , 5μm, 150mm×2.
1mm (id) or equivalent; mobile phase: 0.
1% aqueous acetic acid + acetonitrile (62+38, v/v); flow rate: 0.
3mL/min; column temperature: 35 ℃; Injection volume: 20μL
.

Table 8-32 Retention time, parent ion and product ion of the four analytes

(2) Qualitative determination

Choose one parent ion and two or more product ions for each tested component.
Under the same experimental conditions, the retention time of the substance to be tested in the sample is within ±2.
5% of the corresponding retention time in the matrix mixed standard calibration solution.
Within; and the relative abundance of the qualifier ions of each component in the sample spectrum is compared with the relative abundance of the corresponding qualifier ions in the matrix mixed standard calibration solution spectrum with close concentrations, and the deviation does not exceed the range specified in Table 1-5.
It can be determined that there is a corresponding analyte in the sample
.

(3) Quantitative determination

Under the best working conditions of the instrument, sample the matrix mixed standard calibration solution, and draw the standard working curve with the peak area as the ordinate and the concentration of the matrix mixed calibration solution as the abscissa.
Use the standard working curve to quantify the sample.
The response value of the measured object should be within the linear range measured by the instrument
.

Figure 8-15 Select ion LC-MS-MS of a-Trenbolone, β-Trenbolone, 19-ethylene nortestosterone and epi-19-ethylene nortestosterone standards Figure
1.

Related Links: Determination of a-Trenbolone, β-Trenbolone, 19-Ethylene Nortestosterone and Epi-19-Ethylene Nortestosterone Residues in Cow Urine