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    Home > Professor Li Juan and Professor Yang huanghao of Fuzhou University have made important progress in the field of glycosylation detection of specific proteins in living cells

    Professor Li Juan and Professor Yang huanghao of Fuzhou University have made important progress in the field of glycosylation detection of specific proteins in living cells

    • Last Update: 2019-02-03
    • Source: Internet
    • Author: User
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    Recently, Professor Li Juan and Professor Yang huanghao of the school of chemistry of Fuzhou University have made important progress in the field of glycosylation detection of specific proteins in living cells The research results were published in J am Chem SOC (DOI: 10.1021 / JACS 8b08442) under the title of "amplified visualization of protein specific glycation in zebrafish via proximity induced hybridization chain reaction" The first author is Miss Li Jingying, and the corresponding authors are Professor Li Juan and Professor Yang huanghao As we all know, glycosylation is a kind of complex protein post-translational modification widely existing in eukaryotic cells, which takes part in many important physiological processes The visualization of glycosylation of specific proteins on cell surface is very important to elucidate the function of glycosylation in the dynamic physiological environment of cells In recent years, fluorescence resonance energy transfer (FRET) has been used to detect the glycosylation of specific proteins However, there are many problems in FRET imaging, such as high background signal, low efficiency, and difficulty in selecting the right donor / acceptor fluorescent molecule pair To solve these problems, Professor Li Juan and Professor Yang huanghao designed a proximity induced HCR strategy to amplify glycosylation of specific proteins on the surface of living cells by combining DNA proximity hybridization and hybridization chain reaction (HCR) In this method, the glycosylation of PTK7 protein and EpCAM protein on the surface of living cells was detected by the design of glycosylation probe and protein recognition probe, and the signal amplification of HCR was induced by the adjacent hybridization between the probes, which can sensitively detect the change of glycosylation level of protein under the action of different concentrations of drugs This method is simple and rapid for the specific glycosylation of protein without complex and time-consuming gene engineering modification of the target protein It is suitable for different biological samples, such as living cells, zebrafish, etc., and is expected to be used for the analysis of actual clinical samples Therefore, the new amplification imaging method has a good prospect in the further study of the role of glycosylation of specific proteins in the development of related diseases This work provides a new idea for the development of sensitive and efficient glycosylation detection strategies for specific proteins, especially for real-time dynamic detection in living cells and even in vivo The research work was supported by National Natural Science Foundation of China (No 21622502, 21475026, 21505021, 21605023), natural science foundation of Fujian Province (No 2016j05035, 2017j06004), Changjiang Scholars and innovation team development plan (No irt15r11), etc.
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