Professor Li Xingang's team made new progress in the study of the mechanism of anthoside formation in the "red in the fetal" fruit skin

Original title: Professor Li Xingang's team made new progress in the study of the mechanism of formation of anthocyanins in the "red in the fetal"
Recently, professor Journal of Agricultural and Food Chemistry, a team of professors at the Lin College, published online the title "me
tabolomic and Trans
criptomic Analyses of anthocyanin BiosyntheThe Mechanisms in The Mutant Ziziusjusju Tailihong"s cover article. Dr. Shi Wei of Lin College is the first author of the thesis, and Professor Li Xingang is the author of the communication.
is an important economic fruit tree in China, with a long history of cultivation, a wide range of planting areas, fruit, medicinal food and both by consumers. The fruit is rich in polyphenols and has high nutritional value. "Red in the tire" fruit in the development process, the young fruit fuchsia, the initial stage of maturation is yellow, after maturation is red. Early studies have found that the fuchsia of "red in the fetus" is caused by the accumulation of anthotin. However, the biosynthetic pathways and regulatory mechanisms that affect the accumulation of antholine are not yet clear.
by transcription and metabolic analysis, it was found that cyanidin-3-O-rutinoside, peonidin-3, 5-O-diglucoside and cyanidin were the main components of anthotides during the "red in the fetal" eucalyptus period, which in turn transcription The group was screened to obtain the key synth enzymes ZjANS, ZjUGT79B1 and ZjCCoAOMT, while the key transcription factors ZjMYB5, ZjTT8 and ZjWDR3 were obtained to regulate anthoside synthesis. Through the primary nucleotide expression and in-body enzyme active test, the key role of anthoside synthase anthoensase ZjANS and secondary glycosylation modification enzyme ZjUGT79B1, as well as the gene ZjCCoAOMT, which is responsible for methylation function, was identified. In order to determine the positioning of ZjANS, ZjUGT79B1, ZjMYB5, ZjTT8 and ZjWDR3 in cells, ZjANS-GFP, ZjUGT79B1-GFP, ZjMYB5-GFP, ZjTT8-GFP and ZjWDR3-GFP fusion vectors were constructed and instantaneously converted into the in-cell cells of ben tobacco leaf. It was found that ZjANS and ZjUGT79B1 were located not only on the nucleus but also on the endos endosurgen network, while ZjMYB5-GFP and ZjTT8-GFP were located in the nucleus, and ZjWDR3-GFP had a large number of fluorescent signals in the nucleus and cytoste. The functions of ZjMYB5, ZjTT8 and ZjWDR3 transcription factors in the late structural genes ZjANS and ZjUGT79B1 regulating the biosynthetic pathways of anthoside were verified by GUS dyeing and double luciferase, and the molecular mechanism of the color formation of the fruit skin was revealed. The research system analyzed ZjMYB5, ZjTT8, ZjWDR3 to participate in regulating the synthesis of antholine, and activated the transcription of ZjANS and ZjUFGT79B1 promoters, thus promoting the accumulation of anthocytin during the "red in the fetal" period.
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