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Hengmei program-controlled quantitative sealing machine - a quantitative water quality E.
coli detector @2022 has been updated If necessary, you can click the previous link to understand Hengmei programmed quantitative sealing machine - a quantitative detection water quality Escherichia coli detector @ 2022 has been Updated1、Overview Hengmei program-controlled quantitative sealing machine is electrically driven by intelligent circuit, internal special heating roller, specially used for 51-well quantitative disc and 97-well quantitative disc sealing film, with the use of microbial detection reagents, providing simple, rapid and accurate quantitative detection of total coliform bacteria, Escherichia coli, fecal (heat-resistant) coliforms, enterococci and Pseudomonas aeruginosa; The 51-well and 97-well dishs are semi-automated quantification methods designed based on the traditional method maximum possible number (MPN) statistical model, which can quantitatively detect water sample results, which can replace and outperform traditional (membrane, multi-tube fermentation) methods for the detection of microorganisms
in water.
Second, the scope of application: Hengmei program-controlled quantitative sealing machine is used for rapid detection of Pseudomonas aeruginosa, enterococcus, total coliform bacteria and fecal Escherichia coli, Escherichia coli, and the total number of colonies in water samples, and can be carried in the wild, emergency and quantitative detection
.
3.
Product description: The enzyme substrate detection device is composed of four parts: program-controlled quantitative sealing machine, 51 or 97-well quantitative detection plate, 100mL quantitative bottle, and enzyme substrate detection reagent, and the range of water samples is detected: drinking water, source water, bottled water, reclaimed water, secondary water supply, pipe network water, wastewater, food water, animal husbandry water, medical water, etc
。 Advantages: The enzyme substrate method is GB5750-2006 included in the standard method for the detection of E.
coli, the enzyme substrate method is the current method for the detection of E.
coli in water, which is currently recognized by the domestic testing department
with its advantages of convenience and speed, low false positives, and rapid detection of a large number of samples 。 Compared with multi-tube fermentation and membrane method, the detection steps of enzyme substrate method are greatly reduced, and the requirements for the experimental environment are not high, the detection time can be reduced to 24 hours, and it has a good application prospect in daily water sample monitoring and emergency monitoring, which can be detected in time, prevented, and minimized the probability of major public safety accidents, taking GB5750-2006 total coliform determination as an example, comparing the three methods, as shown
in the table 。 Table 1 Comparative statistical items / methods of multi-tube fermentation method, membrane method and enzyme substrate method Multi-tube fermentation method filter membrane method enzyme substrate method detection time 3-5 days 2-3 days 1 day false positive—23-26%0.
01 detection range 2-1600MPN/100mL—1-2419.
6MPN/100mL environmental requirementsClean laboratoryClean laboratoryClean laboratoryNo special requirementsExperimenters are required to have a professional foundation, skilled operation with professional foundation, and simple training to quantify standard MPN Table Direct Counting MPN Table Quantification Method 15 Tube Colony Count 51 or 97 Well Plate Method Advantages: 1.
No need to operate
in a sterile room.
2.
Manual operation time is less than 1 minute
.
3
.
No need for medium preparation and sterilization of large quantities of glassware.
4.
Qualitative and quantitative analysis can be completed in 24 hours without verification test
.
MMO-MUG medium conforming to GB5750-2006 "Standard Test Method for Drinking Water" fixed substrate technology enzyme substrate method is equipped with a program-controlled quantitative sealer and packaged with (51-well quantitative tray) or (97-well quantitative tray) for detecting water samples
.