Progress has been made in the development of free amino acid monitoring sensors
Last Update: 2021-02-28
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free amino acids are small molecules of important metabolites in animals, and changes in the concentration of specific types or amino acids are of great significance to the evaluation of animal body nutrition and health status. At present, the detection of free amino acids is mainly achieved by liquid chromatography, amino acid analyzers and other instruments, equipment is expensive, the need for amino acids to be derived treatment, and difficult to achieve dynamic monitoring. In recent years, the development of biosensors has developed rapidly, and the development of implantable and wearable biosensors has become a cutting-edge hot spot. Biosensors for amino acid detection have also been developed, but there are problems such as unstable stability and biosysorbability to be improved. The construction of biosensors of peptide-adapted body (peptide-fitting body) is one of the ways to solve these problems gradually.
The Research Center for Agricultural and Livestock Composite Ecology and Livestock Health And Breeding of the Institute of Subtropical Agricultural Ecology of the Chinese Academy of Sciences used bioinfic information technology to form a protein bank associated with amino acid metabolism by collecting 29185 amino acid metabolic-related proteins from pigs, humans, mice and rats; The peptide bank of 850,000 octalptides was used, and the initial screening of peptides with specific binding force of 20 amino acids was obtained on the scale calculation platform by AOTODOCK software, and 653 octalides with single amino acid specific binding were obtained, with an average of 33 amino acids. Subsequently, the effect of the simulation binding of polypeptides and amino acids was verified using isothermal titration calorimeter to obtain peptide fitting with specific amino acid recognition ability. The construction of electrochemical impedance sensors was carried out by using the verified peptide fittings (for example, specific identification of arginine) and the amino acids were detected and found that the biosensors constructed had good linearity with arginine concentrations in the range of 0.1pM-0.1μM, y=704.5log (c) and 11329.0, (R2 -0.9925), in the serum sample testing has also been good results. (Bio Valley Bioon .com)
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