Progress has been made in the study of biosynthesis of per-semistere.

The erythroponine-type esters have many medicinal activities, many of these compounds have strong anti-cancer effects, these compounds in plants usually only a few parts of the content, such a low level of synthesis limits the development and utilization of this compound, but because of its significant medicinal value, some of these compounds are still used in clinical anti-cancer trials, such as people from the Mediterranean region of toxic carrots to isolate the toxic carotene (Thapsigargin), its derivatives have been developed into prostate cancer treatment drugs.
the internal ester group in the molecular structure of this compound is its active group and determines its medicinal value.
the compounds can be divided into two categories according to the internal ester structure: C12, 6 and C12, 8 nesters.
, scientists have analyzed the formation mechanism of C12,6 neste groups, but the understanding of the formation of C12,8 neste groups is still blank.
In order to analyze the formation mechanism of C12,8 neste groups, researchers at Wuhan Botanical Garden, Chinese Academy of Sciences, used chrysanthemum plant cyclology as a research material, and solved the mystery of the formation of C12,8 neste groups with the help of transcription sequencing techniques, and found that the active group formation was the use of gimaane compounds. Large root ene A acid as the substrate, in the special cytochrome P450 enzyme CYP71BL6 under the action of its C8 position to form hydroxyl, the hydroxy-based then freely attack the c12 position of the base, after dehydration and eventually form C12,8 enderate groups.
the separation of the CYP71BL6 gene for the co-synthesis of C12,8 endeastophincompounds, in order to explore the possibility of artificial yeast synthesis C12,8 neste, the researchers introduced the CYP71BL6 gene into yeast cells, combined with the introduction of the large root follicle a acid synthesis pathway, found that artificially constructed yeast engineering bacteria can automatically synthesize C12,8 nlythan compounds.
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