Progress in characterization of supramolecular self-assembly in USTC

Recently, the international academic journal Annals of the American Chemical Society published on-line the research results of Liang Gaolin, Professor of the University of science and technology of China, entitled alkaline phosphorase triggered simple hydrography and chemiluminescence, which reported the new progress in the characterization of supramolecular self-assembly (J Am Chem Soc., DOI: 10.1021/jacs.6b11041）。 In recent years, there are many methods to characterize the self-assembly process of supramolecules, such as high performance liquid chromatography, Fourier transform infrared spectroscopy, circular dichroism, rheology, fluorescence spectroscopy, nuclear magnetic resonance and so on Among them, the most commonly used characterization method is fluorescence spectroscopy However, fluorescence signal has the disadvantages of photobleaching, background signal and spontaneous fluorescence Compared with the fluorescence signal, the chemiluminescence signal does not need to be excited, which eliminates the interference of background signal and spontaneous fluorescence, and has a higher signal-to-noise ratio Liang Gaolin's research group cleverly designed a system of one enzyme and two substrates, using alkaline phosphatase to act on two substrates at the same time (one substrate for supermolecule self-assembly and one substrate for chemiluminescence), so that the two processes of supermolecule self-assembly and chemiluminescence can be produced at the same time Through experiments, they find out the suitable substrate ratio At this ratio, the gel point and the peak value of chemiluminescence of the supramolecular self assemble glue arrive simultaneously, so that the gelation process of the supramolecular self-assembly is accurately characterized by chemiluminescence signal Progress in characterization of supramolecular self-assembly in USTC