Progress in quality control of traditional Chinese medicine activity screening

Recently, the researchers of Changchun Institute of Applied Chemistry of Chinese Academy of sciences have carried out innovative research on the activity screening, structure characterization and quality control of traditional Chinese medicine by means of modern mass spectrometry and other analytical techniques, and made a series of important progress With the gradual integration of traditional Chinese medicine into the international pharmaceutical market, China's traditional Chinese medicine industry is facing great opportunities and challenges Many traditional Chinese medicine products can not meet the international market because of the weak basic research, unclear material basis and active ingredients to play the efficacy, and nonstandard quality control standards Therefore, relying on modern science and technology, deeply mining the scientific connotation of the treasure house of traditional Chinese medicine, making clear the active ingredients of its efficacy, establishing the quality control and evaluation methods related to biological activity, making the traditional Chinese medicine truly safe, effective and quality controllable, and accelerating the modernization of traditional Chinese medicine are the key problems to be solved in the current Chinese medicine industry and scientific research Through innovative research, researchers have established a screening method for active components of traditional Chinese medicine based on the interaction between biological target molecules such as enzymes and nucleic acids and components of traditional Chinese medicine, and established a mass spectrometry method for simultaneous determination of enzyme inhibitory activity and antioxidant capacity of traditional Chinese medicine for the first time The affinity ultrafiltration mass spectrometry method was established to screen and characterize the active components of traditional Chinese medicine at the same time This method can be used to screen the active components from the resource pool of traditional Chinese medicine for single or different biological target molecules, which is helpful to clarify the mechanism of multi-component and multi-target action of traditional Chinese medicine In addition, the electrospray ionization mass spectrometry (ESI MS) was successfully applied to study the interaction between flavonoids and double stranded and three strand DNA, and the close relationship between the structure of the compounds and the binding ability of DNA was found In the aspect of quality control and evaluation methods of traditional Chinese medicine, chemometrics method was used to combine the data of mass spectrometry / chromatography with the results of activity determination, and a scientific and effective comprehensive quality control and evaluation method of traditional Chinese medicine was established This method not only has strong overall characteristics and can realize quantification, but also has the advantages of easy identification, sensitivity and convenience of main components At the same time, the method has been successfully used in many aspects, such as variety identification, variety optimization, origin, growth period identification, processing, compatibility mechanism research and so on In the study of the characterization method of the composition structure of traditional Chinese medicine, the new method of laser enhanced in source cracking established by the researchers has been successfully used in the structure confirmation and isomer differentiation of neutral oligosaccharides The new method can not only determine the linkage sites of sugars, but also provide a -, β - configuration differences A new substrate for oligosaccharide analysis with high sensitivity and good salt tolerance was also found for the first time Relevant projects are supported by a number of National Natural Science Foundation, National Science and technology support plan and key science and technology development plan of Jilin Province During the implementation of the project, 91 papers have been published, 62 of which are included in SCI; 25 invention patents have been applied, 18 of which have obtained patent certificates; 15 doctoral students and 10 postgraduate students have been trained The project also won the first prize of 2012 Jilin science and technology progress award.