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The
protein
produces red products in alkaline solutions that work with hyalphates (or subbrominates) and α-phenols. This is due to the characteristic reaction of arginine in protein molecules. Many derivatives of nicotine, such as acetic acid and nicotine, also occur in this reaction. Arginine is the only positive
amino acid
, with a sensitivity of 1:250,000.
the Principle
. Protein react with sodium bromate solution α-naphthol solution in alkali condition to produce red component. This is a special reaction of arginine's carbamidine, which is useful in quantiting and determining the natures of protein and the amino acid. The reaction is show as below.
can be oxidized by sodium subbrominate to produce nitrogen. Under the slow action of sodium subbrominate, the non-colored substances continue to oxidize, causing the color to disappear, so excessive sodium subbrominate adversely reacts. The addition of strong urea, destroy excessive sodium subbrominate, can increase the stability of color. This reaction can be used to qualitatively identify proteins containing arginine and quantify the content of arginine.
reagent . Protein solution: same as double urine reaction
2.sodium bromate solution
3.10% NaOH solution
4.0.2% α-phenol solution
5.0.01% anaesthetic solution
1. Add 1 ml of protein solution to
test tube
, add 10% NaOH solution 0.5 ml, 0.2% α-phenol 2 drops, mix and then add 2 drops of sodium bromate solution, observe the phenomenon
2. Take 0.01% arginine solution 1 ml, observe the phenomenon according to the above operation reagent
1.Protein solution
2.S bromodium solution
3.10% NaOH solution
4.0.2% α-naphthol solution
5. 0.01% arginine solution
1.1ml of
protein solution is added into a test tube, then 0.5 ml of 10% NaOH solution and two drops of 0.2% α-naphthol are added. After mixing, 2 drops of sodium bromate solution are added, and observe then the phenomenon.
. 2.Add 1 ml of 0.01% arginine solution to the same procedure above, then observe the phenomenon.
.