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. Experimental Principle: In weak acid conditions (pH5-7),
protein
or
amino acids
andtritone co-heating, can produce blue-purple shrink.this reaction is common to all proteins and α-amino acids (sub-amino acids such as proline and hydroxy proline produce yellow
compounds
).also occurs in other compounds containing amino substances.first step:
Step 2:
Reagents 1. Protein samples: (same as double-shrink reaction)
2.0.1% glycine solution Liquid: (same as double-shrink reaction)
3.0.2% three ketone solution
"Experimental method
test tube
2, respectively, added protein samples and 0. 1% glycine solution 1 ml, then each add 3 ketone solution 0.5 ml, mixed in a boiling water bath
heat
for several minutes, observe the phenomenon, record the results and explain the reason. .
