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    Home > Biochemistry News > Biotechnology News > Protein-DNA Binding Affinities by Capillary Electrophoresis

    Protein-DNA Binding Affinities by Capillary Electrophoresis

    • Last Update: 2020-11-30
    • Source: Internet
    • Author: User
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    Described in this chapter is a capillary electrophoresis (CE) method for the accurate determination of protein-
    DNA
    binding constants (
    1
    ). The advantages of the method include the fast separation and quantitation of protein-DNA complex and free DNA, in uncoated capillaries without sieving matrices, coupled with LIF detection of fluorescently-labeled DNA at n
    M
    to π
    M
    concentrations.
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