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Protein Microsequencing

 Last Update: 2021-01-17
 Source: Internet
 Author: User

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Large-scale and high-throughput approaches play an essential role in the study of the highly complex biological systems. In plant biology, obtaining the complete genome sequence of the model plant
Arabidopsis thaliana
has definitively encouraged the incorporation of a completely new set of tools for the identification, characterization, and quantification of its proteome. Proteomics is a new branch of the protein sciences that makes use of these tools and the set of experimental methods specifically designed for them. In this chapter, we describe a complete method for the isolation, fractionation, and identification of a set of proteins obtained from
Arabidopsis thaliana
cells, stressing methodological aspects that could be of potential interest.

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Preparation of Blotted Membrane for Protein Microsequencing

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Protein Microsequencing

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Preparation of Blotted Membrane for Protein Microsequencing