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    Home > Biochemistry News > Biotechnology News > Protein precipitation reaction - heavy metal salt precipitation protein.

    Protein precipitation reaction - heavy metal salt precipitation protein.

    • Last Update: 2020-10-19
    • Source: Internet
    • Author: User
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    .solution pH is more than the
    protein
    and other electrical points, heavy metal salts (e.g. Pb2 plus, Cu2 plus, Hg2 plus and Ag plus etc.) can easily be combined with proteins into insoluble salts and precipitated.of heavy metal salts is usually quite complete, so heavy metal salts are commonly used to remove proteins from liquids. However, it should be noted that when using certain heavy metal salts (such as copper sulfate or lead acetate) to precipitate proteins, do not overdo it, otherwise it will cause precipitation to dissolve again.

    Protein solution (same as double-shrink reaction)
    2.% CuSO4 solution
    3.3% AgNO3 solution
    Experimental Operation 1. Take
    test tubes
    2 protein solutions 1 ml2. Add 2-3 drops to each tube plus 5% CuSO4 solution and 3% AgNO3 solution to observe the precipitation generated by each tube. . 3. In the test tube of copper sulfate to produce protein precipitation, pour out most of the precipitation, leave a small amount of precipitation, continue to add 5% CuSO4 solution, observe the dissolution of precipitation. .
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