Protein precipitation reaction - organic acid precipitation protein.

Principles:alkaloids are alkaline substances with significant physiological effects in plants - nitrogen-containing alkaline substances. Any substance that can precipitate alkaloids, or that can react with alkaloids in color, is called
alkaloid
. Such as tantalum acid, bitter acid and phosphorus tungsten acid.when
pH of the protein
solution is lower than its isoelectronic point, the protein is cation, which can be precipitated by binding to the anions of the alkaloid reagent. Proteins in the solution can also be precipitated
organic
acid, of which triclostic acid is the most sensitive and specific, so it is widely used to precipitate proteins.Reagents1. Protein solution (same as double-shrink reaction)
2.10% tacrychloroac acid solution
3.20% sulphate solution
(experimental operation1. Take 1 ml of protein solution in
tational tube
and add a few drops of tricloste acetic acid solution to observe the phenomenon; 2. Take 1 ml of protein solution in the test tube, add a few drops of water poplar sulfonate solution, observe the phenomenon.
.