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Protein Science Research Platform Bioimaging Center has made new progress in the development of in-situ cryo-electron microscopy technology

 Last Update: 2021-08-03
 Source: Internet
 Author: User

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On July 8, 2021, the international authoritative journal in the field of structural biology, " Journal of Structural Biology ", published online the technical innovation results "VHUT- cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography", aiming at the technical bottleneck of in-situ structural biology research of tissue samples, developed the VHUT-cryo-FIB, This is another technological innovation after the frozen aqueous section preparation technology of cell samples developed by the center in 2016 ( Journal Structural Biology , 2016, 194:218-222), which expands the research objects of in-situ structural biology from single cells to A tissue sample that is closer to the physiological state

Cryo-electron tomography (cryo-ET) is an important cryo-electron microscopy technique that can obtain in-situ three-dimensional high-resolution ultrastructures of cell and tissue samples, in-situ structural information of biological macromolecules, and proteins Machine interaction information in situ

This technical research is based on the latest cryo-focused ion beam technology (cryo-FIB), designed and developed a set of cryo-transmission hardware, which effectively reduces the vibrating section technology, high-pressure freezing technology, cryo-trimming technology and cryo-focused ion beam thinning The combination of technologies and innovative cryo-focused ion beam cutting technology has formed a complete and efficient technical process VHUT-cryo-FIB for the preparation of frozen water-containing sections of tissue samples

Researcher Sun Fei is the corresponding author of the paper.

This work was funded by the National Natural Science Foundation of China, the Key R&D Program of the Ministry of Science and Technology, and the Beijing Municipal Science and Technology Commission

Figure 1.

Article link: https://#s0075

(Contribution: Scientific Research Platform Bioimaging Center)

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