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Biological macromolecules such as proteins and nucleic acids in cells can form a droplet-like membraneless structure through liquid-liquid phase separation (LLPS), which provides compartmentalized reaction space for various life activities and improves the efficiency of various biochemical reactions
Current studies have proved that LncRNA plays an important regulatory function in cell signal regulation
On July 15, 2021, Lin Aifu's research group from the School of Life Sciences, Zhejiang University published a research paper entitled "A Phosphatidic Acid-binding LncRNA SNHG9 Facilitates LATS1 Liquid-liquid Phase Separation to Promote Oncogenic YAP Signaling" in Cell Research
The study found a series of LncRNAs highly expressed in breast cancer through transcriptome sequencing and bio-signal analysis.
When using immunofluorescence to observe the subcellular localization of the LATS1 protein in the SNHG9 overexpression cell line, the researchers found that similar to the spots formed by the separation of the protein, the LATS1 protein forms droplet-like bright spots in the cytoplasm, suggesting that LATS1 is in this condition.
In summary, this study puts forward a working model for SNHG9 to participate in Hippo signal transduction: PA-bound SNHG9 forms a complex with LATS1 to prevent MOB1 from interacting with LATS1 and inhibit the kinase activity of LATS1; on the other hand, the PrLD sequence of LATS1 protein can Driving the occurrence of LATS1 phase separation, SNHG9 acts as a scaffold to recruit LATS1 protein to aggregate and increase the local concentration of LATS1 protein, promote the occurrence of LATS1 phase separation and inhibit its kinase activity, thereby promoting the nuclear and transcription activity of YAP
This research has been strongly supported by the National Natural Science Foundation of China, Zhejiang University and other projects
Original link: https://