Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC

Profiling of hippocampal peptides by reversed-phase HPLC (RP-HPLC) was used as a tool for identifying molecular events associated with neuro-degeneration following global cerebral i ischemia . Global cerebral ischemia is known to cause impairment of working memory, but not reference memory in humans and rats (
1
,
2
). Histologically, global cerebral ischemia results in the selective, delayed neurodegeneration of hippocampal CA1 pyramidal neurons in humans and rats (
1
,
3
). Quantitatively, there is no significant decrease in the number of CA1 pyramidal cells on post-ischemic d 1, but highly significant decreases on d 3 and 7 (
3
). Consequently, the unique, delayed-onset, and selective vulnerability of post-ischemic hippocampal CA1 neurodegeneration in the four-vessel occlusion (4-VO) rat model of transient, global cerebral i ischemia have become of intense interest for clarification of the mechanisms of neurodegeneration and pathophysiology of amnesia after global i ischemia , as well as identification of molecular targets for pharmacological intervention and treatment (
4
).