Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt
Last Update: 2020-11-06
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Advances in the techniques required for the X-ray microanalysis of cryo-fixed, naturally hydrated plant tissues in the cryo-scanning electron microscope have reached the stage that accurate, cell-specific localization and quantification of the nutrient and toxic elements can be achieved. Advances are described in the successive processes of cryo-fixation, cryo-planing to produce flat surfaces, monitored minimal etching to reveal cell outlines, coating with aluminum, spectrum collection, and quantification by comparison with comparable frozen standard solutions of the elements.
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