Quantitative Proteomic Analysis of Mammalian Organisms Using Metabolically Labeled Tissues
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Last Update: 2020-12-30
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Source: Internet
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Author: User
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Metabolic labeling of mammalian organisms with stable isotopes can be used to provide tissue-specific internal standards foruse in quantitative proteomic analyses. This method provides an alternative and complementary strategy to covalent modificationapproaches using isotope-coded mass tags. This chapter will focus on the generation of the isotope-labeled tissues, the analysisof the sample using Multidimensional Protein Identification Technology, and the computational analysis of the mass spectrometricdata acquired.
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