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    Home > Biochemistry News > Biotechnology News > Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications

    Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications

    • Last Update: 2021-02-28
    • Source: Internet
    • Author: User
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    The invention of real-time polymerase chair reaction (
    PCR
    ) has revolutionized the quantification of gene expression and
    DNA
    copy number measurements. However, after the first documentation of real-time PCR in 1993 (
    1
    ), it took several years for this method to become a mainstream tool. PCR generates DNA copies in an exponential way. As soon as resources are exhausted, however, the so-called plateau phase of PCR reaction is reached, making quantification very unreliable. Therefore, quantification appears most reliable in the early exponential phase of PCR (i.e., in a “real-time” fashion). To ensure measurements in this phase of the PCR cycle, real-time PCR measures as soon as the threshold of detection is definitely reached. The cycle of PCR at which this occurs is then named the threshold cycle (
    2
    ) (
    see
    Fig. 1 ). It is the objective of this chapter to describe the possibilities of TaqMan real-time PCR for mRNA and DNA quantification and to discuss pitfalls and alternatives.
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