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    Home > Chemicals Industry > Chemical Technology > Rapid detection of clenbuterol (immune colloidal gold method)

    Rapid detection of clenbuterol (immune colloidal gold method)

    • Last Update: 2022-03-15
    • Source: Internet
    • Author: User
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    The scientific name of Clenbuterol is Clenbuterol (CIenbUterol HydrochIoride), commonly known as Clenbuterol , which is a β-adrenergic receptor agonist and is clinically used to treat bronchial asthma, chronic bronchitis and emphysema
    .

    Because the drug can increase lean meat rate, reduce fat deposition and promote animal growth, it is used as a breeding promoter by some animal husbandry companies
    .
    As a feed additive, the dosage of clenbuterol far exceeds the clinical dosage by 5-10 times.
    After humans consume poultry products produced by animals fed with clenbuterol as an additive, the residual clenbuterol can cause Food poisoning usually causes symptoms such as muscle tremor, palpitations, nervousness, headache, muscle pain, dizziness, nausea, vomiting, fever, and trembling.
    In severe cases, it can cause death and pose a great harm to consumer health

    .
    Many countries in the world have food poisoning cases caused by the consumption of Clenbuterol residues.
    Therefore, many countries prohibit the addition of Clenbuterol in feed to increase the lean meat rate of animals

    .

    [Detection principle]

    Two invisible lines are fixed on the central membrane surface of the Clenbuterol rapid detection reagent plate.
    Clenbuterol antigen is fixed in the test area as the detection line (T line), and the secondary antibody is fixed in the quality control area as the control line (C line).
    )

    .

    When the sample solution to be tested is dropped into the sample hole of the reagent plate, the sample solution diffuses upward due to the chromatographic effect
    .
    If the sample solution contains the residue of the corresponding drug, the drug will react with the antibody on the colloidal gold particles first.
    Therefore, when the colloidal gold particles diffuse to the T line with the sample solution, the active sites of the antibody on the colloidal gold particles will be affected by the sample solution.
    The drug occupies and cannot bind to the drug antigen on the T line; therefore, when the content of clenbuterol in the sample reaches 1 μg/kg or more, the T line on the reagent plate will be lighter than the C line or even no color, and it is judged as Positive

    .
    Conversely, when the content of clenbuterol in the sample is below 1μg/kg or there is no residue, the color of the T-line on the reagent plate is similar to or darker than the C-line, and it is judged as negative

    .

    [Instrument and Reagents]

    (1) Clenbuterol Hydrochloride Immunocolloidal Gold Rapid Detection Reagent Plate (40 strips/box)

    (2) Straw (1pc/bag)
    .

    (3) Desiccant (1 tablet/bag)
    .

    (4) PBST buffer (1 bottle/box)
    .

    [Operation process]

    (1) Add about 5g of pork tissue sample that has been cut or homogenized at high speed into a 5mL centrifuge tube, and the tube cap is tightly closed
    .

    (2) Heat the centrifuge tube containing the sample in a water bath above 90°C for 10 minutes
    .

    (3) After the heating is completed, take it out and cool it to room temperature.
    It is obvious that there is solution seeping from the bottom

    .

    (4) Pipette 3 drops (about 100 uL) of the boiled sample exudate into a 1.
    5 mL centrifuge tube

    .

    (5) Pipette 3 drops (about 100uL) of clenbuterol hydrochloride tissue sample special dilution into a 1.
    5mL centrifuge tube and mix well

    .

    (6) Take out the test card from the aluminum foil bag and lay it flat
    .
    Pipette 3 drops (approximately 100 mL) of the mixed solution into the sample hole of the reagent plate

    .

    (7) Start timing after the sample liquid is dropped into the sample hole, and the result will be judged for 5 to 10 minutes
    .

    [Result judgment]

    As shown in Figure 5-2
    .

    Figure 5-2 Judgment of Clenbuterol Rapid Test Results

    (1) Negative (-) T line (test line, one end near the sample hole) is deeper or as deep as C line (control line), which means that the concentration of clenbuterol in the sample is less than 1μg/kg or does not contain gram of hydrochloric acid Lentro remains
    .

    (2) positive (+) T line is shallower than the C line, or no T-line color indicates the sample hydrochloride Clenbuterol concentrations above 1μg / kg; T more shallow compared to the line C-line, g denotes the sample hydrochloride The higher the concentration of Lenterol
    .

    (3) Invalid No C line appears, indicating that the operation process is incorrect or the reagent plate has deteriorated and is invalid
    .
    In this case, read the instructions carefully again and retest with a new reagent plate

    .

    [Precautions]

    (1) The reagent plate taken out of the original packaging bag must be used as soon as possible within 1h after opening
    .

    (2) Try not to touch the white membrane surface in the center of the reagent plate
    .

    (3) Do not use expired reagent plates
    .

    Related Links: Sensory Evaluation of Meat and Meat Products

     

     

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