Rapid unlabeled identification of brain tumor biopsy tissue using near-infrared Raman spectra and fluorescence spectra

In the past few decades, the application of neuroimaging, brain function positioning, neuronavigation and fluorescence technology has improved the recognition of malignant brain tumors, and can expand the scope of tumor removal while retaining nerve functionIn recent years, Raman Spectroscopy optical molecular imaging technology has developed rapidly as a new non-invasive, unmarked real-time diagnostic method for identifying the pathological images of tumor biopsy histologyRoberta Galli of the Department of Anesthesiology and Critical Medicine at the University of Dresden, Germany, and others assessthe diagnostic value of near-infrared Raman spectroscopy and fluorescence spectroscopy for brain tumor biopsy tissue, and the results are published online in Frontiers inoncology in November 2019the method of study
the researchers analyzed 209 cases of brain tumors in 209 cases of untreated fresh and fresh tissueFreshly untreated biopsies are quickly tested after being fed into the Raman spectroscopy system for a pipeline of isoperated physiological saline solutionsresultsresults show that the classification of biopsy tissue based on Raman spectroscopy and fluorescent signaling techniques can be obtained through the main component analysis, distinguishing non-tumor tissue, tumor tissue and tumor entitiesBased on the pathological examination of tissue slicing, 7 cases of non-tumor biopsy tissue and 195 cases (97%) of tumor biopsy tissue were correctly identifiedThe accuracy of primary GBM was 94%, and the recognition accuracy of recurrent GBM was 100%The correct recognition rates of astrocytosma and glioblastoma were 86% and 90%, respectivelyBrain metastatic tumors, meningiomas and neurosis can all be correctly identifiedIn addition, the correct rate of identification of glioma stoma and metastatic tumor was 90%There were differences in the total deletion of 1p/19q of glioblastoma and IDH1 mutant astrocyma, with a correct recognition rate of 81%conclusionsauthors point out that Raman spectroscopy is a pureoptical technique that allows rapid, unlabeled analysis of brain tumor biopsiesThe study showed that in real-time surgery, Raman spectroscopy can complete a pathological diagnosis of the tumor in a matter of minutes and is highly accurate and sensitive In the case of pre-unknown tumor types, the identification of brain tumors with normal tissue, as well as the extraction of diagnostic-related information, can help to develop further treatment options.