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Rat total complement (CH50) enzyme-linked immunoassay (ELISA)

 Last Update: 2020-11-23
 Source: Internet
 Author: User

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< "font-SIZE: 14.0pt >; > rat Total Supplement (CH50)"< FONT-SIZE: 14.0pt;"> Enzyme-linked immunoanalytic analysis(ELISA)

ReagentsBox Instructions for Use

reagents are for research purposes onlyPurpose: This kit is used to determine rats< < a href" > serum, plasma and related liquid samples < "FONT-SIZE: 12.0pt; COLOR: black;" > total complement (CH50)activity.

< ""> Experimental Principle:

" This kit applies a doubleantibody clamping method to determine the rats in the specimentotal complement (CH50)level. With purified rats"color;" > total complement (CH50)""> antibody package ismicroplate, made solid phase antibodies, packed with mono-resistant micro-holes in turn added"color: > the total complement (CH50), and then with ">HRP < "" >span style < " color: black; "> total complement (CH50) "" antibody binds to form an antibody - antigen - ase-labeled antibody complex, after thorough washing of the substrate TMB color display. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The depth of the color is positively related to the total complement (CH50) in the sample. Measure the absorbent at 450nm < ""> wavelength" ( OD < "" >"), using a standard curve to calculate the concentration of rats in the sample total complement (CH50)" .

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